Mouse Monoclonal Antibody to CAPN1
货号：
31705
别名：
CANP; muCL; CANP1; SPG76; CANPL1; muCANP
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P07384
规格：
目录价
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Description |
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The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
References |
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| 1.Cell Physiol Biochem. 2018;46(2):451-460. 2.Cell Cycle. 2017 Mar 19;16(6):574-577. |
Specification |
|
|---|---|
| Aliases | CANP; muCL; CANP1; SPG76; CANPL1; muCANP |
| Entrez GeneID | 823 |
| Swissprot | P07384 |
| clone | 5B2G10 |
| WB Predicted band size | 81.9kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CAPN1 (AA: 501-714) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using CAPN1 mouse mAb against Jurkat (1), K562 (2), MCF-7 (3), and PC-3 (4) cell lysate.
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Flow cytometric analysis of Hela cells using CAPN1 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CAPN1 mouse mAb with DAB staining.
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