Mouse Monoclonal Antibody to HIST2H3C(27Ac)
货号:
30901
别名:
H3; H3.2; H3/M; H3F2; H3FM; H3FN
应用:
WB,IHC,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q71DI3
规格:
目录价
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Description |
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. |
References |
|---|
| 1.Cell Cycle. 2014;13(3):440-52. 2.Cell Cycle. 2009 Jun 1;8(11):1747-53. |
Specification |
|
|---|---|
| Aliases | H3; H3.2; H3/M; H3F2; H3FM; H3FN |
| Entrez GeneID | 126961 |
| Swissprot | Q71DI3 |
| clone | 6E7A9 |
| WB Predicted band size | 15.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human HIST2H3C (AA: ATKAARK(Ac)SAPATGGV). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

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Western blot analysis using HIST2H3C(27Ac) mouse mAb against Hela (1), Lncap (2), Jurkat (3), and Jurkat (4) cell lysate.

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Flow cytometric analysis of Raji cells using HIST2H3C(27Ac) mouse mAb (green) and negative control (red).

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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using HIST2H3C(27Ac) mouse mAb with DAB staining.

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Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using HIST2H3C(27Ac) mouse mAb with DAB staining.






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