Mouse Monoclonal Antibody to HPRT1
货号:
30677
别名:
HPRT; HGPRT
应用:
WB,IHC,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P00492
规格:
目录价
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Description |
|---|
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. |
References |
|---|
| 1. Nucleosides Nucleotides Nucleic Acids. 2011 Dec;30(12):1248-55. 2. Mol Ther. 2010 Jan;18(1):54-62. |
Specification |
|
|---|---|
| Aliases | HPRT; HGPRT |
| Entrez GeneID | 3251 |
| Swissprot | P00492 |
| clone | 5F11A7 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

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Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.

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Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).

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Immunohistochemical analysis of paraffin-embedded human kidney tissues using HPRT1 mouse mAb with DAB staining.





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