Mouse Monoclonal Antibody to VIL1
货号：
30700
别名：
VIL; D2S1471
应用：
WB,IHC,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P09327
规格：
目录价
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Description |
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This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. |
References |
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| 1. Cancer Sci. 2012 Aug;103(8):1493-501. 2. Cancer Biol Ther. 2011 Aug 1;12(3):181-90. |
Specification |
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|---|---|
| Aliases | VIL; D2S1471 |
| Entrez GeneID | 7429 |
| Swissprot | P09327 |
| clone | 3E5G11 |
| WB Predicted band size | 92.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human VIL1 (AA: 1-209) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using VIL1 mouse mAb against SW620 cell lysate.
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Immunofluorescence analysis of Hela cells using VIL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of SW620 cells using VIL1 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using VIL1 mouse mAb with DAB staining.
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